Short Tandem Repeats Human

Short tandem repeats strs the human genome is full of repeated dna sequences. Short tandem repeat polymorphisms strps are small tracts of tandemly repeated dna sequences that are polymorphic and generally of high heterozygosity in the human population. The dna sequence of a representative allele of this locus is shown below.

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The tandem repeat content of mammalian genomes has been investigated in several papers generally confining the analysis to intergenic regions and or assuming the repeat element is repeated many times 8 14 reports on tandem repeat sequences in human exons have found that almost all repeats have a period unit size that follows the codon size i e.

Short tandem repeats human. These repeated sequences come in various sizes and are classified according to the length of the core repeat units the number of contiguous repeat units and or the overall length of the repeat region. The tetrameric repeat sequence of d7s280 is gata. This sequence comes from genbank a public dna database. A period of 3 6 or 9 bp 8 13 15.

Short tandem repeat str typing methods are widely used today for human identity testing applications including forensic dna analysis. The tandem repeat content of mammalian genomes has been investigated in several papers generally confining the analysis to intergenic regions and or assuming the repeat element is repeated many times 8 14 reports on tandem repeat sequences in human exons have found that almost all repeats have a period unit size that follows the codon size i e. Following multiplex pcr amplification dna samples containing the length variant str alleles are typically separated by capillary electrophoresis and genotyped by comparison to an allelic ladder supplied with a commercial kit. This dna is found on human chromosome 7.

Different alleles of this locus have from 6 to 15 tandem repeats of the gata sequence. A period of 3 6 or 9 bp 8 13 15. By designing pcr primers to locus specific telomeric strps the sequence across the repeats can be amplified and the allele sizes measured.