Tandem Quadrupole Time Of Flight Mass Spectrometry

Mass resolving power of about 10 000 fwhm and accuracy in the range of 10 ppm are observed in both single ms mode and ms ms mode. The mass spectrometer was operated in positive mode and the parameter settings were as follow. Drying gas flow 10 l min 1.

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Q tof ms instrumentation closely resembles that of a triple quadrupole mass spectrometer though the third quadrupole has been replaced by a time of flight tube.

Tandem quadrupole time of flight mass spectrometry. Tandem mass spectrometry also known as ms ms or ms 2 is a technique in instrumental analysis where two or more mass analyzers are coupled together using an additional reaction step to increase their abilities to analyse chemical samples. The q tof ms is a hybrid instrument combining quadrupole technologies with a time of flight mass analyser. Shevchenko a 1 loboda a shevchenko a ens w standing kg. A brief introduction is presented to the basic principles and application of a quadrupole time of flight tof tandem mass spectrometer.

Efficient and targeted profiling of flavonoids in scutellaria barbata. Capillary voltage was set at 4000 v. A quadrupole time of flight hybrid tandem mass spectrometer. Sheath gas flow and temperature 11 l min 1 and 350 c respectively.

A powerful tool for proteomic research. Flavonoid aglycone oriented data mining in high performance liquid chromatography quadrupole time of flight tandem mass spectrometry. 1 european molecular biology laboratory embl heidelberg germany. A matrix assisted laser desorption ionization maldi source has been coupled to a tandem quadrupole time of flight qqtof mass spectrometer by means of a collisional damping interface.

Gas temperature 300 c. Maldi quadrupole time of flight mass spectrometry. The main features of reflecting tof instruments with orthogonal injection of ions are discussed. Nebulizer pressure 50 psig.